Cloning and Characterisation of Chlorophyll Synthase from Avena sativa

Cloning and characterisation of chlorophyll synthase from Avena sativa.

The chlorophyll synthase gene from oat (Avena sativa) was cloned and expressed in Escherichia coli. The deduced amino acid sequence consists of 378 amino acids including a presequence of 46 amino acids. Deletion mutants show that a core protein comprising amino acid residues 88 to 377 is enzymatically active. The sequence of the mature protein shows 85% identity with the chlorophyll synthase of...

Electrophoresis and Electrofocusing of Phytochrome from Etiolated Avena sativa L

R udolf Schendel and W olfhart Rüdiger Botanisches Institut der Universität München Z. Naturforsch. 44c, 12—18 (1989); received November 25, 1988 Microheterogeneity, Pest Sequence, Phytochrome Denaturation, Protein Conformation Phytochrome from etiolated oat seedlings (Avena sativa L.) was investigated by “native” gel electrophoresis and by isoelectric focusing. At pH 8 . 8 the Pfr form migrate...

Assessment Report on Avena Sativa L., Herba and Avena Sativa L., Fructus

Metabolic Changes in Avena sativa Crowns Recovering from Freezing

Extensive research has been conducted on cold acclimation and freezing tolerance of fall-sown cereal plants due to their economic importance; however, little has been reported on the biochemical changes occurring over time after the freezing conditions are replaced by conditions favorable for recovery and growth such as would occur during spring. In this study, GC-MS was used to detect metaboli...

Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii

Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitr...